b type natriuretic peptide  (Elabscience Biotechnology)

Journal: Basic Research in Cardiology

Article Title: Empagliflozin restores cardiac function in obese-diabetic HFpEF mice but further alters gene expression

doi: 10.1007/s00395-026-01184-7

Figure Lengend Snippet: Empagliflozin regulates the multiorgan HFpEF phenotype in obese-diabetic mice. A Study protocol of empagliflozin treatment and assessment of multiorgan impairments, cardiac function, and cardiomyocyte excitation–contraction (EC) coupling. Empagliflozin (Empa) was administered in vivo for 4-wks in leptin receptor (Lepr)-deficient db/db mice with chronic aldosterone infusion ( db/db + Aldo) that induced heart failure with preserved ejection fraction (HFpEF) and in vehicle-treated wild-type mice (WT + Veh). B Blood glucose, body mass, plasma B-type natriuretic peptide (BNP), and pulmonary edema (quantified as wet-to-dry lung weight) in Empa-treated HFpEF mice. Kruskal–Wallis test with Dunn’s multiple comparison’s test. C Cardiac hypertrophy (quantified as heart weight to tibia length ratio, HW/TL), hepatomegaly, kidney weight, and sarcopenia (quantified as combined gastrocnemius and soleus muscle weights) in HFpEF. Welch ANOVA followed by Dunnett’s T3 multiple comparison’s test. The number of animals is shown in the figure

Article Snippet: B-type natriuretic peptide (BNP) levels were measured in blood plasma by ELISA (RayBiotech, EIAM-BNP-1).

Techniques: In Vivo, Clinical Proteomics

Journal: Cardiology Research

Article Title: Nuclear Respiratory Factor-1 Ameliorates Heart Failure by Suppressing Cardiomyocyte Pyroptosis-Associated Signaling Via the Downregulation of Gasdermin D and Caspase-1

doi: 10.14740/cr2153

Figure Lengend Snippet: NRF-1 is negatively correlated with pyroptosis in HF patients. (a) Representative images of echocardiography in HF patients and NF controls. (b–g) Serum NT-proBNP, IL-18, IL-1β, NRF-1, GSDMD and caspase-1 level in HF patients (n = 15) and NF controls (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001. GSDMD: gasdermin D; HF: heart failure; IL: interleukin; NF: normal cardiac function; NRF-1: nuclear respiratory factor-1; NT-proBNP: N-terminal pro-B-type natriuretic peptide.

Article Snippet: ELISA kits were used to detect N-terminal pro-B-type natriuretic peptide (NT-proBNP, EK1393, Multi Science, Hangzhou, China), NRF-1 (E15022h, EIAab, Wuhan, China), IL-18 (EK118S, Multi Science, Hangzhou, China), IL-1β (EK101B, Multi Science, Hangzhou, China), gasdermin D (GSDMD) (E15480h, EIAab, Wuhan, China), and caspase-1 (E1592h, EIAab, Wuhan, China), following the instructions of manufacturers.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: A swine model of severe chronic thromboembolic pulmonary hypertension induced by repeated pulmonary artery long suture injection

doi: 10.3389/fcvm.2025.1736958

Figure Lengend Snippet: Right ventricular myocardium gene expression (A) and protein levels (B) , and circulating plasma mediator levels (C) in chronic thromboembolic pulmonary hypertension (CTEPH) and sham (sham). Gene expression data depicts genes involved in myofilament remodeling (MYH6 and MYH7, α- and β-myosin heavy chain isoforms, respectively), extracellular matrix fibrosis (COL1A1 and COL3A1, type I and III collagen chains, respectively), Ca 2+ -handling (ATP2A2, sarcoendoplasmic reticulum calcium ATPase 2; RYR2, cardiac ryanodine receptor), and neurohumoral mediators (NPPB, B-type natriuretic peptide; EDN1, endothelin-1; TNF, tumor necrosis factor-α). Data were averaged upon normalization for 2 internal control genes, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and RPL4 (ribosomal protein L4). Calcium-handling protein levels of sarcoendoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) normalized for GAPDH and phospho-phospholamban (PLB) normalized for total PLB and representative Western blot bands are presented in (B) , whilst mediator plasma levels of N-terminal pro–B-type natriuretic peptide (NT-proBNP) and endothelin-1 (ET-1) are presented in panel C. In panels A and B data are presented relative to Sham reference levels (dashed lines). * P < 0.05 vs. Sham by Student's t -test or Mann–Whitney U -test, according to assumptions ( n = 6 and 7 in Sham and CTEPH, respectively).

Article Snippet: Previously aliquoted plasma samples were thawed on ice and assayed for N-terminal pro–B-type natriuretic peptide (NT-proBNP; CSB-EQ027465PI, Cusabio) and endothelin-1 (ET-1; ADI-900-020A, Enzo Life Sciences Inc) in duplicate according to the manufacturer's instructions.

Techniques: Gene Expression, Clinical Proteomics, Control, Western Blot, MANN-WHITNEY